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Microfluidic devices with chemical reaction circuits.

Formation of N-branched oligonucleotides as by-products in solid-phase oligonucleotide synthesis

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Q., Expanded utility of the native chemical ligation reaction.

In the past, oligonucleotide synthesis was carried out manuallyusing, as containers for the solid phase, miniature glass columnssimilar in their shape to low-pressure chromatography columns orsyringes equipped with porous filters.Currently, solid-phase oligonucleotide synthesis is carried outautomatically using computer-controlled instruments(oligonucleotide synthesizers) and is technically implemented incolumn, multi-well plate, and array formats. The column format isbest suited for research and large scale applications where ahigh-throughput is not required. Multi-well plate format isdesigned specifically for high-throughput synthesis on small scaleto satisfy the growing demand of industry and academia forsynthetic oligonucleotides. A number of oligonucleotidesynthesizers are available commercially.

Living Radical Polymerization as a Tool for the Synthesis of Polymer-Protein/Peptide Bioconjugates.

The authors note that the detailed studies of the molecular mechanisms of DNA repair pathways were made possible by using site-specifically modified oligonucleotides and that the availability of phosphoramidites to synthesize oligonucleotides with DNA lesions has contributed to the field. They illustrate the article using primarily structural studies in the following examples:

Solid-phase oligonucleotide synthesis - ATDBio

Stereochemistry and functional groups effect on the reaction mechanisms

B., Click chemistry in situ: Acetylcholinesterase as a reaction vessel for the selective assembly of a femtomolar inhibitor from an array of building blocks.

V.; Van der Eycken, E., A microwave-assisted click chemistry synthesis of 1,4-disubstituted 1,2,3-triazoles via a copper(I)-catalyzed three-component reaction.

and seminars on solid-phase chemical synthesis of oligonucleotides

M., Synthesis of bio-inspired hybrid polymers using peptide synthesis and protein engineering.

AB - We have designed and fabricated a microfluidic reactor array device for massively parallel in situ synthesis of oligonucleotides (oDNA). The device is made of glass anodically bonded to silicon consisting of three level features: microreactors, microchannels and through inlet/outlet holes. Main challenges in the design of this device include preventing diffusion of photogenerated reagents upon activation and achieving uniform reagent flow through thousands of parallel reactors. The device embodies a simple and effective dynamic isolation mechanism which prevents the intermixing of active reagents between discrete microreactors. Depending on the design parameters, it is possible to achieve uniform flow and synthesis reaction in all of the reactors by proper design of the microreactors and the microchannels. We demonstrated the use of this device on a solution-based, light-directed parallel in situ oDNA synthesis. We were able to synthesize long oDNA, up to 120 mers at stepwise yield of 98%. The quality of our microfluidic oDNA microarray including sensitivity, signal noise, specificity, spot variation and accuracy was characterized. Our microfluidic reactor array devices show a great potential for genomics and proteomics researches.

Phosphoramidites that allow the generation of oligonucleotides containing site-specific lesions have been vital components for studying the mechanism of DNA repair. New DNA lesions are still being discovered and the study of their biological consequences will require their site-specific incorporation into oligonucleotides. The authors conclude that the increased availability of phosphoramidites for the synthesis of lesion-containing oligonucleotides should facilitate many future discoveries in the broad area of DNA damage and repair.

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  • Oligonucleotide synthesis is the chemical synthesis of ..

    Syntheses of Hemoprotein Models that can be Covalently Attached onto Electrode Surfaces by Click Chemistry.

  • The Chemical Synthesis of DNA and RNA Oligonucleotides …

    S., Surface reactions on demand: electrochemical control of SAM-based reactions.

  • ChemInform Abstract: Chemical Synthesis of Oligonucleotides by ..

    J., Synthesis and Evaluation of Coumermycin A1 Analogues that Inhibit the Hsp90 Protein Folding Machinery.

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for the chemical synthesis of oligonucleotides

Glen Research is delighted to introduce a GalNAc modification strategy using a monomeric GalNAc support and the equivalent GalNAc phosphoramidite. Our experimental work has shown that these products are fully compatible with regular oligonucleotide synthesis and deprotection. Oligonucleotides containing GalNAc can be deprotected using standard procedures during which the acetyl protecting groups on the GalNAc group are removed. Glen Research offers these GalNAc C3 products under an agreement with AM Chemicals LLC.

Apparatus for the chemical synthesis of oligonucleotides ..

The core of our program in phosphorus-based chemical biology focuses on the mechanism-based design, synthesis and evaluation of biologically active molecules such as enzyme inhibitors, and the development of novel antisense oligonucleotides with emphasis on the application of automated synthesis and combinatorial techniques whenever possible.

Chemical Synthesis of Oligonucleotides;

N2 - We have designed and fabricated a microfluidic reactor array device for massively parallel in situ synthesis of oligonucleotides (oDNA). The device is made of glass anodically bonded to silicon consisting of three level features: microreactors, microchannels and through inlet/outlet holes. Main challenges in the design of this device include preventing diffusion of photogenerated reagents upon activation and achieving uniform reagent flow through thousands of parallel reactors. The device embodies a simple and effective dynamic isolation mechanism which prevents the intermixing of active reagents between discrete microreactors. Depending on the design parameters, it is possible to achieve uniform flow and synthesis reaction in all of the reactors by proper design of the microreactors and the microchannels. We demonstrated the use of this device on a solution-based, light-directed parallel in situ oDNA synthesis. We were able to synthesize long oDNA, up to 120 mers at stepwise yield of 98%. The quality of our microfluidic oDNA microarray including sensitivity, signal noise, specificity, spot variation and accuracy was characterized. Our microfluidic reactor array devices show a great potential for genomics and proteomics researches.

1,2-Amino Alcohols and Their Heterocyclic Derivatives …

The use of synthetic oligonucleotides and their mimics to inhibit gene expression by hybridizing with their target sequences has been hindered by their poor cellular uptake and inability to reach the nucleus. Covalent postsynthesis or solid-phase conjugation of peptides to oligonucleotides offers a possible solution to these problems. As feasible chemistry is a prerequisite for biological studies, development of efficient and reproducible approaches for convenient preparation of peptide−oligonucleotide conjugates has become a subject of considerable importance. The present review gives an account of the main synthetic methods available to prepare covalent conjugation of peptides to oligonucleotides.

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