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Acetyl—CoA synthetase - Wikipedia

T1 - The source of acetyl coenzyme A for acetylcholine synthesis in the perfused rat phrenic nerve-hemidiaphragm

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Acetyl—CoA synthetase or Acetate ..

The bifunctional enzyme carbon monoxide dehydrogenase/acetyl-coenzyme A (CoA) synthase (CODH/ACS) is a key enzyme in the Wood−Ljungdahl pathway of carbon fixation. Carbon monoxide is combined with a methyl group and ultimately converted to acetyl-CoA at a unique Ni-containing bimetallic site in the A-cluster of this enzyme. Despite years of extensive biochemical and spectroscopic studies and the recent report of three separate crystal structures, the mechanism by which acetyl-CoA is synthesized is still unknown. Over the past two years there have been a number of significant developments regarding ACS. This Account critically examines these recent developments and especially focuses on those areas that are still a matter of debate.

that make up Acetyl CoA synthetase are acetate and coenzyme A ..

The thioester link, however, is very high energy bond, and therefore unstable. This means that the acetyl group can be easily transferred to other molecules, and so acetyl-CoA is used as a universal intermediate which provides the C2 fragment for numerous biochemical syntheses.

Acetyl-coenzyme A synthesis from …

T1 - Acetyl-coenzyme A synthesis from methyltetrahydrofolate, CO, and coenzyme A by enzymes purified from Clostridium thermoaceticum

A novel coupling system, which is an acetylation system of primary alcohols with acetyl coenzyme A [acetyl-CoA] formed via the metabolism of glucose and acetate, was developed with Hansenula and Pichia. The supplementation of sodium acetate to glucose as the source of acetyl-CoA was effective to enhance the reaction rate and yield of acetylation of citronellol.

Eric Hegg received his B.A. in Chemistry and History from Kalamazoo College in 1991 and his Ph.D. in Chemistry from the University of Wisconsin-Madison in 1996 with Professor Judith Burstyn. After completing postdoctoral studies with Professor Larry Que, Jr. at the University of Minnesota, Eric joined the faculty at the University of Utah in 1999 where he is now an Assistant Professor of Chemistry. In addition to developing models that mimic the active site and chemistry of acetyl-CoA synthase, his research interests include understanding the biosynthesis and transport of heme A.

Acetylcholine Synthesis and Metabolism | Sigma-Aldrich

Acetyl coenzyme A or acetyl-CoA is a molecule that participates in many ..

The chain lengths of the fatty acids in naturally occurring triglycerides can be from 5 to 28 carbon atoms. However, the natural fatty acids found in plants and animals are usually composed only of numbers of carbon atoms, due to the way they are bio-synthesised from C2 units using acetyl-CoA. But bacteria possess the ability to synthesise odd- and branched-chain fatty acids. Therefore, the fat from ruminant animals ( cows) contains significant proportions of branched-chain fatty acids, due to the action of bacteria in the gut.

AB - Many anaerobic bacteria fix CO2 via the acetyl-coenzyme A (CoA) (Wood) pathway. Carbon monoxide dehydrogenase (CODH), a corrinoid/iron-sulfur protein (C/Fe-SP), methyltransferase (MeTr), and an electron transfer protein such as ferredoxin II play pivotal roles in the conversion of methyltetrahydrofolate (CH3-H4folate), CO, and CoA to acetyl-CoA. In the study reported here, our goals were (i) to optimize the method for determining the activity of the synthesis of acetyl-CoA, (ii) to evaluate how closely the rate of synthesis of acetyl-CoA by purified enzymes approaches the rate at which whole cells synthesize acetate, and (iii) to determine which steps limit the rate of acetyl-CoA synthesis. In this study, CODH, MeTr, C/Fe-SP, and ferredoxin were purified from Clostridium thermoaceticum to apparent homogeneity. We optimized conditions for studying the synthesis of acetyl-CoA and found that when the reaction is dependent upon MeTr, the rate is 5.3 μmol min-1 mg-1 of MeTr. This rate is approximately 10-fold higher than that reported previously and is as fast as that predicted on the basis of the rate of in vivo acetate synthesis. When the reaction is dependent upon CODH, the rate of acetyl-CoA synthesis is ~0.82 μmol min-1 mg-1, ~10-fold higher than that observed previously; however, it is still lower than the rate of in vivo acetate synthesis. It appears that at least two steps in the overall synthesis of acetyl-CoA from CH3-H4folate, CO, and CoA can be partially rate limiting. At optimal conditions of low pH (~5.8) and low ionic strength, the rate-limiting step involves methylation of CODH by the methylated C/Fe-SP. At higher pH values and/or higher ionic strength, transfer of the methyl group of CH3-H4folate to the C/Fe-SP becomes rate limiting.

Coenzyme A biosynthesis - UniProt
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  • Acetyl Coenzyme A - Molecule of the Month May 2007 …

    Acetylcholine is synthesized from acetyl coenzyme A and choline by the enzyme choline acetyltransferase

  • Acetyl Coenzyme A The molecule that ..

    Protein involved in the biosynthetic pathway leading from pantothenate to coenzyme A (CoA)

  • Acetyl Coenzyme A Carboxylase - The Journal of …

    Coenzyme A - Wikipedia

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Coenzyme A: Health benefits, Synthesis, and Function.

AB - A novel coupling system, which is an acetylation system of primary alcohols with acetyl coenzyme A [acetyl-CoA] formed via the metabolism of glucose and acetate, was developed with Hansenula and Pichia. The supplementation of sodium acetate to glucose as the source of acetyl-CoA was effective to enhance the reaction rate and yield of acetylation of citronellol.

acetyl-CoA biosynthetic process | SGD

N2 - A novel coupling system, which is an acetylation system of primary alcohols with acetyl coenzyme A [acetyl-CoA] formed via the metabolism of glucose and acetate, was developed with Hansenula and Pichia. The supplementation of sodium acetate to glucose as the source of acetyl-CoA was effective to enhance the reaction rate and yield of acetylation of citronellol.

Unraveling the Structure and Mechanism of Acetyl-Coenzyme A Synthase

T1 - Synthesis of citronellyl acetate via a transacetylation to citronellol from acetyl coenzyme A produced from glucose and acetate in growing yeasts

Coenzyme-A Technologies Inc. Coenzyme A Product

Acetyl-CoA is also used in the synthesis of isoprenoid molecules, esters and amides (. acetylcholine - an important neurotransmitter), and some steroids, (, cholesterol).

acetyl coenzyme A Study Sets and Flashcards | Quizlet

N2 - Many anaerobic bacteria fix CO2 via the acetyl-coenzyme A (CoA) (Wood) pathway. Carbon monoxide dehydrogenase (CODH), a corrinoid/iron-sulfur protein (C/Fe-SP), methyltransferase (MeTr), and an electron transfer protein such as ferredoxin II play pivotal roles in the conversion of methyltetrahydrofolate (CH3-H4folate), CO, and CoA to acetyl-CoA. In the study reported here, our goals were (i) to optimize the method for determining the activity of the synthesis of acetyl-CoA, (ii) to evaluate how closely the rate of synthesis of acetyl-CoA by purified enzymes approaches the rate at which whole cells synthesize acetate, and (iii) to determine which steps limit the rate of acetyl-CoA synthesis. In this study, CODH, MeTr, C/Fe-SP, and ferredoxin were purified from Clostridium thermoaceticum to apparent homogeneity. We optimized conditions for studying the synthesis of acetyl-CoA and found that when the reaction is dependent upon MeTr, the rate is 5.3 μmol min-1 mg-1 of MeTr. This rate is approximately 10-fold higher than that reported previously and is as fast as that predicted on the basis of the rate of in vivo acetate synthesis. When the reaction is dependent upon CODH, the rate of acetyl-CoA synthesis is ~0.82 μmol min-1 mg-1, ~10-fold higher than that observed previously; however, it is still lower than the rate of in vivo acetate synthesis. It appears that at least two steps in the overall synthesis of acetyl-CoA from CH3-H4folate, CO, and CoA can be partially rate limiting. At optimal conditions of low pH (~5.8) and low ionic strength, the rate-limiting step involves methylation of CODH by the methylated C/Fe-SP. At higher pH values and/or higher ionic strength, transfer of the methyl group of CH3-H4folate to the C/Fe-SP becomes rate limiting.

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